Journal: bioRxiv

Article Title: Acid stress modulates metabolo-inflammatory pathways in oral epithelial cells

doi: 10.64898/2026.03.16.711383

Figure Lengend Snippet: Schematic representation of the experimental design to investigate the impact of acid stress on oral epithelial cells (OECs) and Toll-like receptor (TLR) agonist stimulation. pH Conditioning : OECs (80% confluence) were cultured in acidified (pH:=:3.0) or complete growth media (pH:=:8.0) for 24h. Morphometric Analysis ( a ): Brightfield micrographs and a machine-learning based image analysis pipeline were used to assess changes in cellular morphology. TLR Agonist Challenge : Cell cultures were subjected to either 100 ng/mL flagellin (TLR5 agonist) or 1 mg/mL Pam3CSK4 (TLR2/1 agonist) for 2-, 6-, or 24h. Molecular profiling ( b ): was conducted on OEC RNA collected after 6h TLR agonist challenge using NanoString® nCounter® technology followed by pathway analysis using the Gene Ontology knowledgebase. A TGF- β ELISA ( c ) was performed on OEC supernatants collected 2-, 6-, and 24h post TLR agonist stimulation. Figure made with Biorender.com

Article Snippet: Low-passage mixed-donor, Primary Human Oral Epithelial Cells (Cat# 36063-01, Celprogen, Inc., Torrance, USA), were thawed from liquid nitrogen, plated on poly-L-lysine-coated T-75 cell culture flasks (Sigma-Aldrich Co., St. Louis, USA; Corning Inc., Durham, USA) and maintained in Human OEC Culture Complete Growth Media (Celprogen, Inc.) containing serum and antibiotics at 37°C and 5% CO 2 for two passages prior to experimentation according to manufacturer recommendations.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The Porphyromonas gingivalis RNA-binding protein is required for growth in high levels of zinc and persistence with host cells

doi: 10.3389/fcimb.2025.1569544

Figure Lengend Snippet: RbpPg1 is required for survival of P. gingivalis with host cells. (A) Survival of WT W83 P. gingivalis and V3139 RbpPg1-deficient strain with the immortalized HN4 cell line. Bacterial survival was quantified by counting colony-forming units following lysis of infected cells. MOI used was 100:1. (B) Human oral keratinocytes (HOKs) infected with WT W83, V3128- rbpPg1 mutant, and complemented strain (V3236) at an MOI of 100:1.

Article Snippet: An immortalized oral epithelial cell line, HN4 cells , and primary human oral keratinocytes (HOKs) (ScienCell) were used for our study, and infection was carried out as described previously ( ; ; ; ).

Techniques: Lysis, Infection, Mutagenesis